Came from the medical school of a federal university and has always been very well taken care of. At the heart of the countess systems fluorescence technology lie the unique invitrogen evos light cubes. Countess ii and countess ii fl automated cell counters. The countess ii automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. Cell culture basics handbook invitrogen gibco cell culture basics training certification exam. Ready to use for your conveniencecountess cell counting chamber slides are individually packaged. The countess automated cell counter offers easy and accurate cell counting and viability counts. Countess automated cell counter university imaging centers. For detailed instructions on using the countess automated cell counter, refer to the manual supplied with the counter or download the manual from. Cell sizes, mean diameter, total cell concentration, graphical data presentation and images are some of the parameters that can be determined. Mar 12, 2020 countess ii fl automated cell counter description with the option for a reusable slide and fluorescence capabilitiesbrightfield and two userchangeable fluorescence channelsthe invitrogen countess ii fl automated cell counter can count cells, monitor fluorescent protein expression, and measure cell viability. A cell count was then performed by an automated cell counter countess. Specimens tzmbl, 293s gnti and 293t17 cell lines listed in various protocols.
Your lab is the threshold for your next cell culture breakthrough. The tc20 automated cell counter demonstrates accurate counts of viable cells. Having a cell counter means eliminating the subjectivity and time sink of. Invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. Invitrogen is one of several brands under the thermo fisher scientific corporation. Each slide contains two cell counting chambers 75 mm.
Invitrogen may be used in lieu of a light microscope hemacytometer for cell counting and viability calculation. Power adapter is included with this invitrogen countess cat 10227 cell counter. Accurateuse the countess ii automated cell counter to eliminate the subjectivity of manual cell coun. Thermo scientific invitrogen countess ii fl user manual pdf. Cell culture cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells e. I am trying to use free software flowing software to analyse and produce my.
Our group is looking into getting a countess ii fl cell counter from life technologies link. Designed for use with countess automated cell counter. When hmscs reached 90% confluence, cells were trypsinized. Countess ii fl automated cell counter thermo fisher. Performing a simple brightfield count on the countess ii fl automated cell counter. Countess ii and countess ii fl automated cell counters user guide. Invitrogen countess ii fl automated cell counter with the option for a reusable slide and fluorescence capabilitiesbrightfield and two userchangeable fluorescence channelsthe countess ii fl automated cell counter can count cells, monitor fluorescent protein expression, and measure cell viability in as little as 10 seconds. Description with the option for a reusable slide and fluorescence capabilitiesbrightfield and two userchangeable fluorescence channelsthe invitrogen countess ii fl automated cell counter can count cells, monitor fluorescent protein expression, and measure cell viability. Invitrogen countess cell counting chamber slides quantity. Reportsave a printerfriendly pdf report of the results, graphs, and image figure 2. Labelfree impedance flow cytometry for nanotoxicity. We have 1 thermo scientific invitrogen countess ii fl manual available for free pdf download.
Insert the countess ii usb drive or equivalent into an available usb port on the instrument. The invitrogen countess ii fl automated cell counter offers brightfield counting and two optional, user changeable fluorescence channels. Cell counter thermo scientific countess ii with counting time of 10 seconds, for cell concentrations of 10,000 to 10,000,000 cellsml, cell diameters of 7 to 60um, 5mp image resolution, pdf report format, stores 10 protocols, with touch screen and usb port, no thermal printer, not suitable for fluorescence. Countess ii automated cell counter quick reference card 1 each the countess ii automated cell counter is a fully automated cell counter and assay platform that uses stateoftheart optics and image analysis algorithms to analyze trypan bluestained cells in suspension. Invitrogen countess ii fl automated cell counter from. The invitrogen countess ii and countess ii fl automated cell counters contain advanced autofocusing and counting algorithms to allow you to quickly and accurately identify and count cells within a population while avoiding user variation associated with manual focusing mechanisms. The measurement range extends from 1 x 10e4 to 1 x 10e7 cellsml the optimal range is 1 x 10e5 to 4 x 10e6 cellsml, which is broader than that of a. Countess ii automated cell counter thermo fisher scientific. Countess ii and countess ii fl automated cell counters quick reference card qrc. The result was expressed as the number of cells per square centimeter of cell culture surface cellscm 2. The countess automated cell counter offers an intuitive user interface, and provides the option to save and print cell count data using the countess software download from vitrogen. May 26, 2009 the countess automated cell counter from invitrogen is a benchtop automated cell counter. The countess ii fl automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts.
Invitrogen was founded in 1987 by lyle turner, joe fernandez, and william mcconnell and was incorporated in 1989. Allow at least 5 cm 2 in free space at the back of the instrument to allow for. The guides listed below are available with the countess ii or the countess ii fl automated cell counter. A 7the countess automated cell counter is designed to read samples from 1. This listing is for a perfect working condition invitrogen countess cat 10227 cell counter as can be watched in the video and also seen in the pictures. It can perform cell counts as well as viability and cell calculations. Used invitrogen countess c10227 cell counter for sale. The countess is selfcontained so you do not need an additional. The reuseable slide option for this cell counter means cutting down on consumables cost significantly. Tc20 automated cell counter life science research biorad. For viability measurements using cultured cells at 1 x 106 cellsml, the countess instrument. Refurbished invitrogen countess automated cell counter.
The invitrogen countess ii automated cell counter uses trypan blue staining combined with an autofocus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts in as little as 10 seconds. The tc20 automated cell counter uses microscopy with autofocus that analyzes multiple focal planes to identify the best plane. But nothing counts like countess ii automated cell counters. Count cells, measure cell viability and monitor fluorescent protein expressionin just 10 seconds with no slides or tips. Invitrogen countess ii fl automated cell counter countess ii fl.
Select the desired files to save your experiment result, images, data. Invitrogen countess ii fl automated cell counter starter. I count them anyways and proceed to validate my results with the tc20 automated cell counter by biorad and confirm that they show similaridentical percentage of live vs dead cells. Countess automated cell counter from invitrogen biocompare. Countess ii fl automated cell counter from thermo fisher. How do these systems generally perform in comparison with manual counting using coulter chambers. The countess automated cell counter from invitrogen is a benchtop automated cell counter.
Instructions for using the countess test beads for verifying the proper functioning of the countess automated cell counter is described below. The complete instruction manual is available on our website at. On the results screen, press save to go to the save screen. The countess has a touch screen display and allows data to be transferred via usb drive to a computer for data access and storage. Countess instrument was highly correlated with that of the hemocytometer.
The cell counting algorithm then uses the image acquired from the best focal plane to identify cells and exclude debris, thereby calculating the total cell count. The product line includes various subbrands of biotechnology products, such as machines and consumables for polymerase chain reaction, reverse transcription, cloning, culturing, stem cell production, cell therapy, regenerative medicine, immunotherapy, transfection, dnarna purification, diagnostic tests. A 30 seconds per sample q what is the counting range. Slides and glass countess ii fl reusable slides on. The invitrogen countess automated cell counter uses trypan blue stain to stain cells for counting and viability studies. User manual thermo scientific invitrogen countess ii fl user manual 82 pages. Thermo scientific invitrogen countess ii fl manuals. The company initially found success with its kits for molecular cloningnotably, the librarian, a kit for making cdna libraries, and the fasttrack kit for mrna isolation from biological samples. Accurateuse the countess ii automated cell counter to eliminate the subjectivity of manual cell counting and usertouser variability. The tc20 counter and hemocytometer cell counts showed no statistically significant differences. You can get all the data you need about your cell cultures in just 30 seconds without using a hemocytometer. This set includes 100 boxes of slides 50 slides per box and 2 100 ml bottles of trypan blue 0.
Dilution calculatornew builtin dilution calculator displays volume of cell sample and buffer needed figure 1. Read the safety data sheets sdss and follow the handling. Label free impedance flow cytometry for nanotoxicity screening. Simplified workfloweasily save protocols from the adjustments screen. For use at room temperature under normal laboratory conditions.
This software allows you to manipulate your cellcounting data and to save your data in a pdf report format for printing. For safety and biohazard guidelines, refer to the safety appendix in the countess ii automated cell counter user guide pub. Invitogen cell countess nov252009 we just got the new cell countess from invitrogen and was wondering if any one else is using this equipment for cells shldbcrzy. A serumfree medium formulation efficiently supports.
The countess from invitrogen uses slides that require loading 10 ul in each side 20 ul for the whole slide of cell solution. With threechannel flexibilitybrightfield and two optional fluorescence channelsresearchers can count cells, monitor fluorescent protein. Invitrogen countess ii fl automated cell counter countess ii. The countess automated cell counter faqs basic technical questions q how long does it take to count. Summary of contents for thermo scientific invitrogen countess ii fl page 1 countess ii fl automated cell counter user guide for fluorescence and brightfield applications catalog number amqaf document part number mp10826 publication number man0010644 revision e.
Invitrogen countess ii automated cell counter fisher. Invitrogen countess automated cell counter manual st. Your updated countess ii or countes ii fl instrument will have these added benefits. The countess automated cell counter faqs basic technical questions. Cell yield was assessed by microscopy and cell count. Invitrogen countess ii automated cell counter from thermo. Protein and cell analysis brochure fisher scientific. Light cubes are userinterchangeable and autodetected by the countess ii fl system for plugandplay capability. Formulation of serum free medium in our laboratory. The thermo countess ii fl cell counter also has two optional user changeable fluorescence channels to fit your needs, and comes standard with a brightfield to monitor protein expression and measure cell viability before downstream analysis. Jan 10, 2020 label free impedance flow cytometry for nanotoxicity screening. Review and cite automated cell counter protocol, troubleshooting and other. Download the latest countess iiii fl software version to your desktop. Countess ii automated cell counter catalog number amqax pub.
The new countess ii automated cell counter is a benchtop assay platform equipped with stateoftheart optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. Were here to help image of the front cover of the molecular probes handbook. Countess cell counting chamber slides are designed for use with the countess automated cell counter. Countess automated cell counter from thermo scientific. Cell counter thermo scientific countess ii fl with counting time of 10 seconds, for cell concentrations of 10,000 to 10,000,000 cellsml, cell diameters of 7 to 60um, 5mp image resolution, pdf report format, stores 10 protocols, with touch screen and usb port, no thermal printer, suitable for fluorescence. A wide variety of prokaryotic cell can be counted within a very short time, usually less than a minute per sample. The thermo countess ii fl cell counter brings you reliable cell counting and viability assessment with the added convenience of reusable hemocytometers and the option to add fluorescence capabilities. Each cube contains an led, illuminating optics, and filters. Invitrogen countess cell counting chamber slides print share invitrogen countess cell counting chamber slides. Typically, cells need nutrients organic nutrients such as carbohydrates, lipids, amino acids, and vitamins. Countess ii fl automated cell counter fisher scientific. Countess ii fl automated cell counter the countess ii fl automated cell counter is a fully automated, 3channel cell counter and assay platform that uses evos light cube technology, stateoftheart optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samplesin suspension. Do not press the optical surfaces of the chamber slides.
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